map2k inhibitor pd0325901  (ReproCELL)

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: Granulosa cells collected by follicular puncture from mice treated with PD0325901 showed absence of phosphorylation of Mapk3/1 at Thr302/Tyr204 compared to vehicle treated mice (n = 3 mice/group/time point).

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Phospho-proteomics

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: Treatment of immature mice with single dose of 25μg/g of PD0325901 resulted in anovulation compared to vehicle treated mice (A) during superovulation (n = 5 mice/ group). This anovulation as evidenced by trapped GV stage oocytes with compact cumulus cells (C&D) inside the preovulatory follicles at 18h hCG (n = 5 mice/ group). Vehicle treated mice showed well-developed corpus luteum (B) evidencing follicular rupture, ovulation and luteinization. CL—corpus luteum; GV—germinal vesicle.

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques:

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: Treatment of immature mice with single dose of 25μg/g of PD0325901 did not alter relative mRNA abundance of Mapk1 and Mapk3 (A). Expression of known hCG regulated genes like Fshr and Nr5a2 (B) as well as hCG induced genes like Scarb1 and Pappa (C) were also not altered in PD0325901 treated mice (n = 3mice/group/time point).

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: Treatment of immature mice with single dose of 25μg/g of PD0325901 inhibited the expression of hCG-induced genes involved in follicular rupture like Ptgs2 , Pgr , Adamts1 ; genes involved in cumulus expansion like Has2 , Ptx3 , Tnfaip6 ; gene involved in oocyte meiotic maturation like Areg and luteinization like Cebpb (n = 3mice/group/time point). Mapk3/1 regulated LH induced genes like Ptgs2 , Ptx3 and Tnfaip6 were not regulated by Cebpb.

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: A) Treatment of immature mice with single dose of 25μg/g of PD0325901 inhibited the expression of hCG-induced expression of Egr1 1h and 4h hCG. B) Abundance of Egr1 protein in granulosa cells of the inhibitor and vehicle treated mice. Actb was used as loading control.

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: A) Treatment with PD0325901 decreased Fo+PMA induced increases in the abundance of Ptgs2 protein relative to vehicle treated primary granulosa cells. B) Pre-treatment with Egr1-siRNA inhibited Fo+PMA induced expression of Egr1 when compared to control siRNA treatment in primary granulosa cells in vitro. This was associated with reduced abundance of Pgts2 transcript in Egr1-siRNA treated cells. Fo—forskolin; PMA—phorbol-12-myristate.

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Expressing, Control, In Vitro

Journal: PLoS ONE

Article Title: Effect of the Transient Pharmacological Inhibition of Mapk3/1 Pathway on Ovulation in Mice

doi: 10.1371/journal.pone.0119387

Figure Lengend Snippet: Preovulatory LH surge induced Mapk3/1 activity regulates transcription of ovulatory genes such as Ptgs2 trough transcription factor Egr1. Inhibition of this LH-induced Mapk3/1 activity by Map2k inhibitor PD0325901 results in anovulation.

Article Snippet: A potent selective Map2k (MEK) inhibitor PD0325901 (Selleckchem) was initially dissolved in DMSO (Fisher Scientific) to prepare a stock solution of 100 μg/μl concentration.

Techniques: Activity Assay, Inhibition